Faecal Culture and IS900 PCR Assay for the Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Faecal Samples
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چکیده
منابع مشابه
Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods
Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...
متن کاملDetection of Mycobacterium avium subsp. paratuberculosis in the mesenteric lymph nodes of goats by PCR and culture
The efficacy of bacterial cultures and IS900-specific polymerase chain reaction (PCR) was compared for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) from the mesenteric lymph nodes of goats. Samples were collected from 75 goats slaughtered in Ilam, in southwest of Iran. Tissue homogenates were inoculated onto four media. The genomic DNA was extracted directly from mesenteri...
متن کاملCharacterization of IS900 loci in mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing
Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was char...
متن کاملA biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 elemen...
متن کاملAge at which dairy cattle become Mycobacterium avium subsp. paratuberculosis faecal culture positive Preventive Veterinary Medicine, 97, 29-36 Age at which cattle become faecal culture positive for Mycobacterium avium subsp. paratuberculosis
not available. 865 Kaevska, M., Hruska, K. (2010) Research on Mycobacterium avium during the period 1995 to 2009 Veterinarni Medicina, 55, 473-482 Papers on Mycobacterium avium, published between 1995 and 2009 that are indexed in the databases Web of Science (R) (Thomson Reuters) and PubMed (U.S. National Library of Medicine) were analysed and 3377 papers, published by 11 197 authors from 2630 ...
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ژورنال
عنوان ژورنال: Journal of Pure and Applied Microbiology
سال: 2017
ISSN: 0973-7510
DOI: 10.22207/jpam.11.1.59